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Coding
CD27L
Part:BBa_K3183009:Design
Designed by: David Schramm Group: iGEM19_Oxford (2019-10-14)
CD27L Endolysin Targeting C. difficile Codon-Optimised for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization matched to E. coli codon tables (Kazusa Database)whilst matching all existing RF standards and removing repetitive sequences for easy de novo DNA synthesis.
Source
Protein sequence derived from Mayer et al., 20081. Synthesised as an IDT gBlock.
References
Mayer, Melinda J et al. “Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin.” Journal of bacteriology vol. 190,20 (2008): 6734-40. doi:10.1128/JB.00686-08